IN SILICO AND IN VITRO ANTIDIABETIC ACTIVITY STUDIES ON THE ETHANOL EXTRACT OF DESMANTHUS VIRGATUS (L.) LEAVES

Objective: In vitro and in silico studies using α-glucosidase activity inhibition aimed at identifying the potential antidiabetic properties of ethanol extracts of D. virgatus leaves. Material and Method: Compounds in the ethanol extract of D. virgatus leaves were identified by LC-MS. In vitro and in silico methods were used to determine the antidiabetic activity. Result and Discussion: The protein code (PDB ID) used is 3AJ7 and 3W37, then acarbose was used as a positive control. By using LC-MS,160 compounds were identified from the extract. All of the identified compounds were tested in silico, using 3AJ7 protein 18 compounds and with 3W37 10 compounds were found to fulfill Lipinski\\\'s rule with a binding affinity value ≤ -8.2 kcal/mol for 3A37 protein and ≤ -8.1 kcal/mol for 3W37, which is similar to acarbose. In addition, α-glucosidase inhibition was used to assess the antidiabetic effect of the ethanol extract of D. virgatus leaves in vitro, resulting in IC50 values of 144.11 ppm for the extract and 3.78 ppm for acarbose. The IC50 values of the extract were included in the medium category.