CHARACTERIZATION OF GREEN SYNTHESIZED SILVER AND ZINC NANOPARTICLES USING PROPOLIS EXTRACT AND THEIR ANTIMICROBIAL, ANTIBIOFILM, ANTIQUORUM SENSING ACTIVITIES

Objective: AgNP and ZnO NP were synthesized by green synthesis method using propolis, the characterization of nanoparticles was examined and their antimicrobial, antibiofilm and antiquarum sensing activities were examined. Material and Method: Nanoparticles were synthesized from the prepared propolis extract using 100 ml and 25 ml of extract for AgNP and ZnONP, respectively. Characterization was performed using UV-Visible (UV-VIS) spectra, XRD, EDX and SEM. Antimicrobial, antibiofilm and anti-quorum sensing activities of the nanoparticles were examined. Result and Discussion: Looking at the characterization results, AgNP and ZnO NP with very small particle sizes were synthesized. According to the antibacterial activity results, the MIC values of ZnO NPs and AgNPs were determined as 0.0625 mg/ml and 0.0250 mg/ml against S. aureus ATCC 43300 (MRSA), respectively. The percentage biofilm inhibition value of ZnO NPs and AgNPs was found to be 38.94% and 38.67%, respectively. The synthesized AgNP and ZnO NPs did not show anti-quorum sensing activity.