Identification of bioactive peptides in kashar cheese and its antioxidant activities

This study analyzed the peptide profile and antioxidant activity in commercially sourced Kashar cheese. The antioxidant activity of Kashar cheese was found to be 41.09 mM Trolox g−1. However, the antioxidant activity of its F3, F4, F5 and F14 fractions was found to be 920.726  mM  Trolox g−1,  545.544 mM  Trolox g−1 ,  783.864 mM Trolox g−1,  and 392.12 mM Trolox g−1, respectively. In Kashar cheese, the Tandem Mass Spectrometry insert ignore into journalissuearticles values(MS/MS); spectrum for the 875 g mol−1 m/z signal was matched to αs1-casein, and it showed that 1012 g mol−1 insert ignore into journalissuearticles values(875+137); histidine can be a part of 1140 insert ignore into journalissuearticles values(1012+128); glutamine amino acid. Peptide sequences were matched to 875:RPKHPIK-H-Q peptide 1012:RPKHPIK+H peptide and 1140:RPKHPIKH+Q peptide. It can be concluded that the peptide fractions of Kashar cheese demonstrated antioxidant activity.