Apoptotic Effects of Urtica dioica extract and Boric Acid in MCF-7 Breast Cancer Cell Line: A Study

Authors : Alev Selcan Arköse, Hadi Karimkhani, Gizem Gülmez, Ayşe Esra Karadağ

Pages : 540-549

Doi:10.20515/otd.1580200

View : 41 | Download : 23

Publication Date : 2025-06-18

Article Type : Research Paper

Abstract :This study investigates the effects of Urtica dioica (nettle), Boric acid, and their combinations on MCF-7 human breast cancer cells. The goal is to explore how these substances impact apoptosis (programmed cell death) and anti-apoptotic pathways in a breast cancer model while assessing the protective effects of Urtica dioica and Boric acid.The MTT assay was employed to measure cell viability, and IC50 doses were determined. Protein levels of BAX, p53, Cytochrome C, and Calpain were quantified using the ELISA method. Additionally, phytochemical analyses were conducted using HPLC.Results indicated a statistically significant increase in p53 levels between the control group and those treated with Urtica dioica and Boric acid (p<0.001). Cytochrome C levels also showed a significant increase in the Urtica dioica group, with Boric acid demonstrating a meaningful increase (p<0.001, p<0.01). HPLC analysis of Urtica dioica extract identified caffeic acid as one of the critical phytochemicals.The findings suggest that combining Urtica dioica and Boric acid has limited effects on apoptosis in breast cancer cells, although positive impacts on p53 and Cytochrome C were noted. These results provide a foundation for considering Urtica dioica and Boric acid in breast cancer treatment. However, further clinical and animal studies are needed to evaluate these substances\\\' efficacy, safety, and appropriate dosages. Future research will deepen our understanding of Urtica dioica and Boric acid\\\'s roles in breast cancer therapy and help develop new treatment strategies.
Keywords : Meme kanseri, MCF-7, Isırgan Otu, Borik asit, Apoptoz

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