Determination of Cell Viability, Apoptotic and Necrotic Activities of Boswellia serrata Extract in Liver Cancer (HepG2) and Breast Cancer (MCF7) Cell Lines

Authors : Semih Dalkılıç, Lütfiye Kadıoğlu Dalkılıç, Lütfü Uygur, Ömer Kaya

Pages : 1061-1073

Doi:10.47495/okufbed.1372695

View : 105 | Download : 97

Publication Date : 2024-06-25

Article Type : Research Paper

Abstract :Cancer is a very important disease that has existed for a very long time in the history of humanity and poses a great threat to humanity today. Today, cancer remains a global health problem. The scientific world has introduced many innovative treatment approaches, but due to the complexity of cancer cells and their resistance to treatment, significant progress has been made in the fight against cancer, but a definitive cure has not yet been found. Boswellia serrata has been used in many parts of the world for a variety of ailments from ancient times to the present day, comes from the Burseraceae family and is commonly known as white gum. B. serrata is a resin tree with very small and few leaves, as it is a plant species that grows as a small tree or shrub 4-6 meters high in the desert. In this study, B.serrata resin was obtained commercially in Gaziantep province and obtained by extraction in methanol and hexane solvents. The cytotoxic activity of B. serrata resin extract was determined by MTT Assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Apoptotic/necrotic activity was determined by double staining (Hoechst 33342 and propidium iodide). Human breast cancer (MCF7) and human liver cancer (HepG2) cell lines were used in these experiments.
Keywords : Boswellia serrata, MCF7, HEPG2, Apoptotik, Nekrotik, MTT