Essential Oil Composition, Antioxidant Activity, and Phenolic Content of Melampyrum Arvense L. Var. Arvense L.

Authors : Azize Demirpolat, Duygu Nur Çobanoğlu, Piroz Düzdaban, Dincer Cobanoglu, Muhammad Zafar, Mustaq Ahmad, Ömer Kılıç

Pages : 490-504

Doi:10.47495/okufbed.1496786

View : 158 | Download : 156

Publication Date : 2025-03-12

Article Type : Research Paper

Abstract :In this study, the essential oil composition, antioxidant activity, and phenolic content of Melampyrum arvense L. var. arvense L. were investigated.The aerial part of M. arvense was hydrodistilled, and a light yellowish oil yield of 0.9% (v/w) was obtained. Essential oil composition analysed with GC-MS. Methanolic extracts were prepared from whole parts of M. arvense var. arvense to determine the antioxidant activity and phenolic content was detected with LC-MS/MS. According to GC/MS analysis, 70 compounds were identified in the oil of M. arvense var arvense. The major compounds in M. arvense var. arvense were n-hexadecanoic acid-palmitic (17.73%), phenol, 2,4-bis (1,1-dimethylethyl) (15.56%), and octadecanoic acid-stearic acid (9.96%). Total phenolic and flavonoid content, DPPH•, and ABTS•+ scavenging activities were 33.5±2.6 mg GAE/g dw, 76.5±1.1 mg QE/g dw, 21.5±2.7 mg Trolox/g dw, and 30.7±0.45 mg Trolox/g dw, respectively. The most prevalent phenolic compound was 4-OH-benzoic acid. As a result of this study, high levels of fatty acids were detected in M. arvense var. arvense essential oil, which has not been reported in the previous literature.
Keywords : M. arvense var. arvense, LC-MS/MS, Uçucu yağ, GS-MS, Fenolik, Antioksidan