Decreased GADD45A gene expression level in MGUS

Authors : Ilknur SUER, Aynur DAĞLAR ADAY, Gözde ÖZTAN

Pages : 594-601

Doi:10.31362/patd.1291199

View : 100 | Download : 121

Publication Date : 2023-10-01

Article Type : Research Paper

Abstract :Purpose: Monoclonal gammopathy of undetermined significance insert ignore into journalissuearticles values(MGUS); is a plasma cell dyscrasia. It is known that MGUS has an increased risk of progression to multiple myeloma insert ignore into journalissuearticles values(MM);, and prepares the ground for diseases such as Waldenstrom macroglobulinemia insert ignore into journalissuearticles values(WM);, non-Hodgkin lymphoma, and chronic lymphocytic leukemia insert ignore into journalissuearticles values(CLL);. Our study aimed to evaluate whether some important p53 pathway genes differ in terms of expression between MGUS and healthy individuals. Materials and methods: Bone marrow was collected from eight healthy individuals and eight individuals diagnosed with MGUS, and RNA samples were isolated. The expression levels of various genes involved in the p53 pathway were compared using an RT2-profiler PCR array. β-Actin housekeeping gene expression level was used for normalization. Pearson’s Correlation and Receiver Operating Characteristic insert ignore into journalissuearticles values(ROC); analyses were conducted. Results: Among the genes whose expression levels were examined in this study, it was determined that the expression level of only the GADD45A gene decreased significantly in the MGUS group compared to the control group insert ignore into journalissuearticles values(p=0.027);. Pearson’s correlation data showed that GADD45A gene expression was highly correlated with 12 of the other genes insert ignore into journalissuearticles values(APAF1, CDK4, PCNA, BAX, CDKN2A, CASP9, CHEK2, MDM2, RB1, P53, BCL2, CHEK1); examined in the p53 pathway insert ignore into journalissuearticles values(r>0.7);. In addition, according to the ROC analysis, GADD45A was detected to have strong discrimination power between MGUS and healthy individuals insert ignore into journalissuearticles values(AUC=0.797 and p=0.015);. Conclusion: The decreased expression of the GADD45A gene in the MGUS group compared to the control group may be useful as a new biomarker to detect the development of MGUS.
Keywords : MGUS, p53 yolağı, GADD45A