Impact of cisplatin on Kasumi-1 leukemia cell line: gene expression and DNA damage

Authors : Yavuz Dodurga, Mücahit Seçme, Levent Elmas, Nazlı Demirkıran, Sevda Sağ, Ulviye Adamcı, Zeliha Akdağ

Pages : 63-71

Doi:10.31362/patd.1552452

View : 91 | Download : 141

Publication Date : 2025-01-01

Article Type : Research Paper

Abstract :Purpose: Leukemia is a type of cancer caused by the uncontrolled proliferation of blood cells. The purpose of this study was to investigate the effects of cisplatin (CIS), a chemotherapeutic agent used in the treatment of leukemia, on the Kasumi-1 leukemia cell line. Materials and methods: The study measured the effect of CIS on Kasumi-1 cells by calculating IC50 values for cell viability. The mRNA expression levels of apoptosis and cell cycle-related genes were then assessed using Real-Time PCR. In addition, the effects of CIS on DNA damage were investigated using the comet assay. Results: Significant changes in apoptosis and cell cycle-related genes were observed in CIS-treated groups. These included alterations in the mRNA levels of p53, BCL-2, CHECK 1, CDC25C, CDK 6, URG4/URGCP, GADD45A, CCND1, GADD45G, and ATM genes. Comet analysis confirmed CIS\\\'s effects on DNA damage. Conclusion: This study aimed to better understand how CIS affects genetic mechanisms in leukemia cells and provide new insights into leukemia treatment. The findings will help us better understand the role of CIS in leukemia treatment and will serve as a valuable reference for future research.
Keywords : Lösemi, akut miyeloid lösemi, sisplatin, Kasumi-1 hücresi, DNA hasarı