Expression profiles of LMAN2 in breast cancer cell lines in hypoxia and normoxia conditions

Authors : Ceyda Okudu, Gökhan Ağtürk, Elif Kesim, Ayyub Ebrahımı
Pages : 474-485
Doi:10.31362/patd.1533261
View : 210 | Download : 179
Publication Date : 2025-07-02
Article Type : Research Paper
Abstract :Abstract Purpose: Breast cancer is one of the most common and leading causes of death in women. The mechanism by which breast cancer develops is not fully understood. Understanding the mechanism of initiation and the genes and proteins involved in this process may help us to fight this type of cancer. The LMAN2 gene encodes VIP36, which transports properly folded proteins. The main aim of this study was to investigate the expression of the LMAN2 gene at the molecular level in breast cancer cells with different functional defects. Determining the level of LMAN2 gene expression under hypoxia, known as oxygen deprivation, has a significant impact on tumourigenesis and metastasis, and obtaining new data on the relationship between hypoxia and ER stress was identified as a secondary objective. Material and methods: In this study, the expression level of the LMAN2 gene will be examined in breast cancer cell lines (SKBR3, MDA-MB-231, MDA-MB-468, MCF-7) and CRL4010 cell line as a control. Results: LMAN2 gene expression level was evaluated at 48-hour periods by providing normoxic and hypoxic conditions. While the LMAN2 gene expression level in MCF-7, MDA-MB-231, and SK-BR-3 cells is significantly reduced, it was highly expressed in MDA-MB-468 in hypoxic and normoxic conditions. CHOP, HERP, and BiP gene expression levels were significantly higher in MDA-MB-468 under hypoxic conditions like LMAN2 expression. Conclusion: LMAN2 and other ER stress response elements showed different expression profiles in SK-BR-3, MDA-MB-231, MDA-MB-468, and MCF7 cell lines under hypoxic conditions. Increased expression was found in MCF-7 and MDA-MB-468 cell lines, but decreased expression was detected in SK-BR-3 and MDA-MB-231 cell lines. The underlying reason for this difference is thought to be that the cell lines have different molecular properties, such as triple negative or HER2 (+/-) status.
Keywords : ER stres, hipoksi, LMAN2, meme kanseri, apoptoz

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