Folate production in lactic acid bacteria and determination of folate derivatives by HPLC

Authors : Meryem Nur Zeydanlı, Zehranur Yüksekdağ, Berat Çınar Acar

Pages : 694-700

View : 26 | Download : 9

Publication Date : 2025-08-25

Article Type : Research Paper

Abstract :Folate is a B-group vitamin that humans cannot synthesize and must be taken externally. Although some types of Lactic Acid Bacteria can produce folate, there is insufficient information about the production of folate by yoghurt starter cultures. In this study, four bacteria belonging to two Lactobacillus, and two Streptococcus genera were isolated from yoghurt samples, and the isolates were biochemically identified by 16S rDNA with an API 50 CHL kit. Intracellular and extracellular folate production of the bacteria was designated in various media and buffer applications. The highest intracellular folate production was observed in Lactobacillus delbrueckii subsp. bulgaricus ZN951 (126.3 μg/L) and Streptococcus. thermophilus Z151 (177.1 μg/L) strains in MRS/Elliker medium with potassium phosphate buffer. L. delbrueckii subsp. bulgaricus ZN541 strain showed the highest extracellular folate production (105.1μg/L) in the same medium and buffer, but S. thermophilus Z151 strain exhibited 86.4 μg/L folate productions. Whether the strains contained folate derivatives and pABA was investigated by HPLC analysis and pABA was not detected in any of the strains. Among cultures, the highest total folate content was detected as 110.5μg/L by L. delbrueckii subsp. bulgaricus ZN951 strain. This strain was found to have concentrations of 5-MeTHF and 5-FmTHF. Therefore, the use of these bacteria can be an alternative to naturally increase folate content in foods.
Keywords : ücre içi folat, Hücre dişi folat, Folat türevleri, Laktik asit bakterisi

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