EFFECTS OF SEPERATE OR SIMULTANEOUS INJECTION OF TWO DIFFERENT GENES (ENHANCED GREEN-FLUORESCENCE PROTEIN GENE, HUMAN GAMMA INTERFERON GENE)

Authors : Korhan ARSLAN, Haydar BAĞIŞ, Munis DÜNDAR

Pages : 43-52

View : 23 | Download : 11

Publication Date : 2009-06-01

Article Type : Research Paper

Abstract :hIFN-γ and EGFP gene constructs were separatelyor simultaneously injected to mouse embryos atpronuclear phase. Micro-injection were classifiedin three different experiment groups according toalone or simultaneous transfer of gen constructs. Infirst group co-injection of hIFN-γ+EGFP transgenes were performed. As a result of transfers,totally 39 offsprings were born, In molecularanalysis performed on DNAs obtained from thoseoffsprings, two were EGFP transgenic and one washIFN-γ transgenic. In second group micro-injectionof hIFN-γ trans-gene was performed. As a result oftransfers, totally 21 offsprings were born. Inmolecular analysis performed on DNAs obtainedfrom those offsprings totally two neonates werehIFN-γ transgenic. In third group micro-injection ofEGFP trans-gene was performed. As a result oftransfers, totally five offsprings were born, it wasfound that there were no transgenic offspring.Results presented in present study found thattransgenicity percentage of offsprings born as aresult of simultaneous micro-injection of twodifferent genes was high. If the protocol presentedin this study is applied to transgenic mice recoverystudies, it is possible to recover transgenic micecarrying two different genes separate. Thus, it willbe possible to save money and time
Keywords : Transgen, mikroenjeksiyon, EGFP, hIFN γ, Ko enjeksiyon