The Effects of N-Acetylcysteine on MMP-2 and MMP-9 Immune Activities in Testicular Tissue of Streptezotocin Induced Diabetic Rats

Authors : Alper YALCIN

Pages : 59-67

Doi:10.31832/smj.492802

View : 25 | Download : 11

Publication Date : 2019-03-20

Article Type : Research Paper

Abstract :Objective: This study was performed to investigate the effects of N-Acetylcysteine insert ignore into journalissuearticles values(NAC); on matrix metalloproteinases insert ignore into journalissuearticles values(Mmp-2 and Mmp-9); immunoreactivity in testicular tissue of diabetic rats. Materials and Methods: 28 male rats were allocated into four groups n insert ignore into journalissuearticles values(7);; No treatment was applied to Control group. Diabetes was induced upon injection of a single dose streptozocin 50 mg/kg intraperitoneally on diabetes group insert ignore into journalissuearticles values(DM);. N-Acetylcysteine group insert ignore into journalissuearticles values(NAC); and following  diabetes development, Diabetic + NAC groups were treated with i.p.100 mg/kg NAC daily. Oxidative damage was evaluated with Total Antioxidant Status insert ignore into journalissuearticles values(TAS); and Total Oxidant Status insert ignore into journalissuearticles values(TOS); activities while the testicular damage was determined by histopathological evaluation and immunohistochemical assessment of MMP-2 and MMP-9 at the testicular tissues. Results: TAS levels were found to be increased in diabetic NAC-treated group animals whereas  TOS, MMP-2 and MMP-9 levels were decreased in the same group. For the histological findings, there were no testicular changes In the NAC alone control group whereas the alterations such as marked degeneration, vacuole formation and basement membrane thickening of tubules seminiferus contortus were observed in the testicular tissues of the DM group. But, in the treatment group DM+NAC, these alterations were found to be comparatively decreased. Conclusion:   Our findings suggest that administration of NAC minimize testicular damage in diabetic rats and might be a potential candidate to reduce/eliminate the negative effects of diabetes on the testicular tissue.
Keywords : N acetylcysteine NAC, Mmp2, Mmp9, Testes, Diabetes