Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi

Authors : Esma Hande ALICI, Gülnur ARABACI

Pages : 399-405

Doi:10.16984/saufbed.99926

View : 23 | Download : 9

Publication Date : 2013-06-01

Article Type : Research Paper

Abstract :In this study, Polyphenol oxidase was extracted from Borage plant insert ignore into journalissuearticles values(Trachystemon orientalis); and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5°C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+, Mg2+, Zn2+, Cu2+, Ca2+, K+, but inhibited by Hg2+, Mn2+, Ni2+, Sn2+, Na+. Ba2+, Al3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations.
Keywords : Trachystemon orientalis, Polifenol oksidaz, metal etkisi, enzim kinetiği