Development and Validation of RP-HPLC Method for the Determination of Dexrabeprazole Sodium

Authors : Semra YILMAZER KESKİN, Ebru Nurdan ŞENTÜRK, Cihansel UNLU

Pages : 1427-1431

Doi:10.16984/saufenbilder.999458

View : 21 | Download : 9

Publication Date : 2021-12-31

Article Type : Research Paper

Abstract :A rapid and simple liquid chromatographic method was developed for the quantitative determination of dexrabeprazole sodium in the tablet dosage form. The reverse-phase chromatographic analysis was carried out by using a C-18 column. The acetonitrile-phosphate buffer mixture was used as the mobile phase. The obtained retention time was 4.33∓0.02 min. by using 50:50 insert ignore into journalissuearticles values(v:v); acetonitrile:phosphate bufferinsert ignore into journalissuearticles values(pH 7); and 1.0 mL/min. flow rate. Quantification of the analyte was based on measuring the peaks areas at 272 nm. The analytical performance of the developed RP-HPLC method was validated with respect to accuracy, precision, linearity, stability, and robustness. The obtained linearity range was 77-143 mg/L and the correlation coefficient was 0.9989. The obtained LOD and LOQ values were 0.010 mg/L and 0.034 mg/L, respectively.
Keywords : Dexrabeprazole, RP HPLC, validation