Using Locusta Migratoria as a Nitrogen Source for the Growth and Development of Microorganisms

Authors : Perihan AKBAŞ, Esabi Başaran KURBANOĞLU

Pages : 466-473

Doi:10.16984/saufenbilder.1037496

View : 33 | Download : 7

Publication Date : 2022-06-30

Article Type : Research Paper

Abstract :Characteristics and the use as culture media of protein hydrolysate from Locusta migratoria were determined in comparison with different peptones. After powdering, it was hydrolyzed chemically insert ignore into journalissuearticles values(acid hydrolysis); and obtained product Locust Peptone insert ignore into journalissuearticles values(LP);. The contents of protein, fat, nitrogen, ash, minerals, total sugars and amino acids of LP were determined and it was seen that it has both organic and inorganic materials enough to use as a component of the medium. The effects of different concentrations added 20g/l glucose of LP on the growth of four test bacteria insert ignore into journalissuearticles values(Escherichia coli, Bacillus subtilis, Pseudomonas putida and Staphylococcus aureus); and test yeasts insert ignore into journalissuearticles values(Rhodotorula glutinis, Candida albicans, Saccharomyces cerevisiae); were investigated and it was found that the optimal concentration for bacteria and yeast are %0,6 and LP was compared with bacteriological peptone insert ignore into journalissuearticles values(BP);, fish peptone insert ignore into journalissuearticles values(FP); and meat peptone insert ignore into journalissuearticles values(MP); .The obtained results with surface streaking and shaking culture procedures showed that LP yielded a little higher or equal FP and BP in both normal bacteria, but these values were lower than values obtained from MP. The results show that LP performed similar to or even better than commercial peptones as nitrogen sources for microorganisms growth. A new peptone has been developed from locust for microbiological media in the present study.
Keywords : Culture media, Locusta migratoria, Locust peptone, nitrogen source, peptone, protein hydrolysate