Comparative Evaluation of Alginate-Gelatin Hydrogel, Cryogel, and Aerogel Beads as a Tissue Scaffold

Authors : Ece BAYIR

Pages : 335-348

Doi:10.16984/saufenbilder.1098637

View : 48 | Download : 11

Publication Date : 2023-04-30

Article Type : Research Paper

Abstract :Hydrogels are frequently used in tissue engineering and regenerative medicine, drug delivery, and environmental remediation. Alginate and gelatin, which are frequently used natural polymers to form hydrogels, were chosen in this study to form a core-shell structured hydrogel. Cryogels and aerogels were obtained by drying hydrogels with different methods, freeze-drying, and the continuous flow of supercritical CO2, respectively. The potential use of hydrogels, aerogels, and cryogels as a tissue scaffold was evaluated comparatively. Characterizations of materials were determined morphologically by scanning electron microscope and computed-micro tomography, chemically by energy dispersive spectroscopy, and mechanically by the dynamic mechanical analyzer. In addition, the cytotoxic effect of all structures was analyzed by the WST-1 method and the localization of the cells in these structures was determined by microscopic methods. All scaffolds show non-cytotoxic effects. Cryogels have the highest porosity insert ignore into journalissuearticles values(85.21 %); and mean pore size values insert ignore into journalissuearticles values(62.3±26.8 µm);. Additionally, cryogels show high water retention capacity insert ignore into journalissuearticles values(782±53.5%); than aerogels insert ignore into journalissuearticles values(389±2.5%); for 24 h. The elastic modulus values were <10 kPa, which is suitable for brain, bone marrow, spleen, pancreas, fat, kidney, and skin tissue engineering, for all types of beads. It has been determined that cryogel and hydrogel beads are more suitable for cell adhesion and migration in this study.
Keywords : Tissue engineering, scaffold, sodium alginate, gelatin

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