PROTECTIVE ROLE OF ANKAFERD BLOOD STOPPER ON CADMIUM-INDUCED ACUTE NEPHROTOXICITY

Authors : İlter İLHAN, Halil İbrahim BÜYÜKBAYRAM

Pages : 111-118

Doi:10.17343/sdutfd.1239914

View : 18 | Download : 9

Publication Date : 2023-03-14

Article Type : Research Paper

Abstract :Objective Cadmium insert ignore into journalissuearticles values(Cd); is a very toxic and carcinogenic heavy metal that can cause harmful effects on human health. Toxicity may develop due to Cd exposure, especially in the kidneys. Ankaferd blood stopper insert ignore into journalissuearticles values(ABS); is a herbal mix that is used for its hemostatic properties in surgery. Also, ABS enhances wound and tissue healing. In this study, we aimed to evaluate the possible ameliorative effects of ABS in Cd-induced renal damage. Material and Method Thirty-two male rats were randomly divided into 4 groups: control, Cd insert ignore into journalissuearticles values(cadmium chloride, 2.5 mg/kg single dose, ip);, ABS insert ignore into journalissuearticles values(ABS, 1.5 ml/kg single dose ip);, and Cd+ABS insert ignore into journalissuearticles values(cadmium chloride, 2.5 mg/kg single dose ip- ABS, 1.5 ml/kg single dose ip);. At the end of the experiment, urea and creatinine levels were analyzed from the rats’ serum. In addition, total oxidant status insert ignore into journalissuearticles values(TOS);, total antioxidant status insert ignore into journalissuearticles values(TAS); levels, superoxide dismutase insert ignore into journalissuearticles values(SOD);, and glutathione peroxidase insert ignore into journalissuearticles values(GPx); activity were measured spectrophotometrically from renal tissues. The oxidative stress index insert ignore into journalissuearticles values(OSI); was calculated from TOS and TAS levels. Also, we evaluated alterations in the mRNA expression of Bcl- 2-associated X protein insert ignore into journalissuearticles values(Bax);, B-cell-lymphoma-2 insert ignore into journalissuearticles values(Bcl- 2);, silenced information regulator 1 insert ignore into journalissuearticles values(SIRT1);, and p53 in kidney tissue of rats by using the qRT-PCR method. Results In the Cd group, serum urea, creatinine levels, and tissue oxidative stress markers, TOS and OSI were significantly higher while Gpx activity was significantly lower than in the control group insert ignore into journalissuearticles values(p<0.05);. Also, the expression of p53 and in Bax/Bcl2 ratio significantly increased in the Cd group insert ignore into journalissuearticles values(p<0.05);. But, ABS treatment significantly decreased urea, creatinine, TOS, OSI levels, and Bax/Bcl2 ratio, p53 expression in Cd applied group insert ignore into journalissuearticles values(p<0.05);. Conclusion Ankaferd blood stopper showed protective effects by reducing oxidative stress and mitochondria-mediated apoptosis in acute Cd exposure.
Keywords : Urtica dioica, resveratrol, meyan, kekik, oksidatif stress