ANKAFERD BLOOD STOPPER ALLEVIATES CADMIUM-INDUCED LUNG INJURY BY REDUCING MITOCHONDRIAL STRESS-RELATED APOPTOSIS VIA BAX/BCL-2 AND CYT-C/CAS-3 PATHWAYS

Authors : Muhammet Yusuf TEPEBAŞI, Perihan SEZGİNER

Pages : 225-233

Doi:10.17343/sdutfd.1278783

View : 42 | Download : 41

Publication Date : 2023-06-22

Article Type : Research Paper

Abstract :As a result of environmental factors, cadmium insert ignore into journalissuearticles values(Cd); taken into the body causes damage to lung tissues through inflammation, oxidative stress, and increased apoptosis. Ankaferd Blood Stopper insert ignore into journalissuearticles values(ABS);, which is used as a hemostatic agent, has antioxidant, antiinflammatory, antibacterial, antiapoptotic, and wound healing properties due to five different plant extracts and components in its composition. Therefore, in our study, we aimed to investigate the curative effect of ABS on the toxicity of Cd on the lung. Material and Method Thirty two rats were used in the study, and they were divided into 4 groups, with 8 rats in each group: control, Kd insert ignore into journalissuearticles values(2.5 mg/kg single dose ip);, ABS insert ignore into journalissuearticles values(1.5 ml/ kg single dose ip);, and Kd+ABS insert ignore into journalissuearticles values(Kd, 2,5 mg/kg single dose ip-ABS, 1.5 ml/kg single dose ip);. Lung tissues were evaluated histopathologically. Inflammation was evaluated immunohistochemically with tumor necrosis factor-α insert ignore into journalissuearticles values(TNF-α);. Oxidative stress was evaluated with the total oxidant level insert ignore into journalissuearticles values(TOS); and total antioxidant level insert ignore into journalissuearticles values(TAS); using the spectrophotometric method. Apoptosis was evaluated using RT-PCR with relative mRNA fold changes of Bcl-2-associated X insert ignore into journalissuearticles values(Bax);, B-cell lymphoma 2 insert ignore into journalissuearticles values(Bcl-2);, cytochrome c insert ignore into journalissuearticles values(Cyt c);, and caspase 3 genes. Results Histopathological findings such as congestion, hemorrhage, and mononuclear cell infiltration were found to increase in the Cd group. It was found that Cd increased inflammation by increasing TNF-α, increasing TOS and OSI, and decreasing TAS, causing an increase in oxidative stress. insert ignore into journalissuearticles values(p<0.05);. It was determined that there was a significant improvement in all these parameters after the ABS application insert ignore into journalissuearticles values(p<0.05);. Conclusion In conclusion, we suggested that ABS may be an alternative option to protect against Cd-induced lung toxicity, and more research should be done on this subject.
Keywords : Kadmiyum, Ankaferd Blood Stopper, inflamasyon, oksidatif stres, apoptoz, akciğer