Electrochemical Properties and Determination of Serotonin with Graphene/Coal Tar Pitch/Pencil Graphite Sensor Electrode using Square Wave Adsorptive Stripping Voltammetry Technique

Authors : Şeyma Korkmaz, Hafizullah Sharifi, Ayşen Demir Mülazımoğlu

Pages : 20-27

Doi:10.35238/sufefd.1456420

View : 49 | Download : 43

Publication Date : 2024-04-24

Article Type : Research Paper

Abstract :In the present work, electrochemical and spectroelectrochemical behaviors of Serotonin (5-HT) were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Cyclic voltammetric experiments of 5-HT on graphene/coal tar pitch/pencil graphite electrode (GR/CTP/PGE) were carried out between 0.0 V and 1.8 V potential range at a scan rate of 100 mV s-1 with 20 cycles in non-aqueous media. Surface characterizations were performed using CV, EIS and scanning electron microscope (SEM). Effect of different pH values was investigated by square wave voltammetry (SWV) for determination of 5-HT. Optimization of accumulation time was determined using square wave adsorptive stripping voltammetry (SWAdSV) within potential range of -0.2 to +0.6 V. 5-HT standard solutions changing from 75 µM to 1.0 µM were prepared and the corresponding peak currents were measured. From the obtained data calibration equation was derived Ip = 0.0329C5-HT + 0.1511 with correlation coefficient (R2) 0.9958. LOD was 3.51x10-7 M and LOQ was 1.05x10-6 M.
Keywords : Serotonin, Dönüşümlü Voltametri, Sensör Elektrot, Kare Dalga Adsorptif Sıyırma Voltametrisi