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  • Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Dergisi
  • Volume:3 Issue:1
  • KONYA`DA YETİŞEN CENTAUREA PTEROCAULA TRUATV. `IN FENOLİK YAPISI VE ANTİOKSİDAN ETKİSİ

KONYA`DA YETİŞEN CENTAUREA PTEROCAULA TRUATV. `IN FENOLİK YAPISI VE ANTİOKSİDAN ETKİSİ

Authors : Yener TEKELİ
Pages : 35-41
Doi:10.29233/sdufeffd.134637
View : 18 | Download : 9
Publication Date : 2008-06-01
Article Type : Research Paper
Abstract :antioksidan aktivitesinin belirlenmesi amaçlanmıştır. Centaurea Pterocaula `u yağından uzaklaştırılmak için sokslet apareyi ile petrol eterinde ekstrakte edildi. Sonra materyal %70 lik metanolde tekrar ekstraksiyona tabi tutuldu ve çözücüsünden uzaklaştırıldı. Toplam fenolik madde konsantrasyonu Folin-Ciocalteu metoduna, serbest radikal süpürme etkisi DPPH metoduna göre ve indirgeme gücü ise Oyaizu metoduna göre yapıldı. Bitkinin fenolik yapısı HPLC ile belirlendi. Sonuçlar sentetik antioksidan olan BHT insert ignore into journalissuearticles values(Butillenmişhidroksitoluen); ve BHA insert ignore into journalissuearticles values(Butillenmişhidroksianisol); ile kıyaslandı. Anahtar Kelime: Antioksidan, Centaurea Pterocaula, DPPH, Folin-Ciocalteu, Oyaizu DETERMINATION OF PHENOLIC CONTENT AND ANTIOXIDANT EFFECT OF CENTAUREA PTEROCAULA TRUATV. IN KONYA Abstract: The present study was carried out to determine of Centaurea Pterocaula which was collected in region of Konya Tuz Lake. It was extracted with petroleum ether using a soxhleth apparatus for remove it`s oil. Then the defatted plant materials were extracted with 70% methanol.and concentrated. Total phenol concentration was determined by Folin-Ciocalteu method, free radical scavenging activities were determined based on DPPH and determined reducing power based on Oyaizu method. The phenolic compound of C. Pterocaula was elucidated by HPLC analysis. Results were compared with standard antioxidant compound BHA and BHT. Keywords: Antioxidant, Centaurea Pterocaula, DPPH, Folin-Ciocalteu, Oyaizu
Keywords : Antioksidan, Centaurea Pterocaula, DPPH, Folin Ciocalteu, Oyaizu

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