[N,N`-Bis(salisiliden)-1,3-propandiaminato]nikel(II) Kompleksinin Sentezi ve DNA Etkisinin İncelenmesi

Authors : Mürsel Arıcı, Hasan NAZIR

Pages : 67-74

View : 18 | Download : 12

Publication Date : 2010-06-25

Article Type : Research Paper

Abstract :Özet: Bu çalışmada, bisinsert ignore into journalissuearticles values(salisiliden);-1,3-diaminopropan insert ignore into journalissuearticles values(H2L); Schiff bazı ile nikelinsert ignore into journalissuearticles values(II); klorür hekzahidrat`ın etanoldeki reaksiyonundan [N,N`-bisinsert ignore into journalissuearticles values(salisiliden);-1,3-propandiaminato]nikelinsert ignore into journalissuearticles values(II); insert ignore into journalissuearticles values(NiL); kompleksi sentezlendi. Sentezlenen NiL kompleksinin pBR322 plazmid DNA ile etkileşimi ultraviyole absorpsiyon spektroskopisi insert ignore into journalissuearticles values(UV);, dönüşümlü voltametri insert ignore into journalissuearticles values(CV); ve agaroz jel elektroforez ile incelendi. NiL kompleksinin DNA varlığında absorpsiyon spektrumunun hiperkromik etki gösterdiği ve kaydedilen dönüşümlü voltamogramında pik akımının azaldığı bulundu. NiL kompleksinin indirgeyici maddeler olmadan plazmid DNA`yı etkilediği agaroz jel elektroforez ile gösterildi. Ayrıca NiL kompleksinin Hyperchem® programıyla hesaplanan en düşük enerjili yapısının geometrisi, DNA`nın kısmi interkalasyon ve elektrostatik bağlama modlarıyla ilişkilendirilebilir bir sonuç vermektedir. Anahtar kelimeler: Nikelinsert ignore into journalissuearticles values(II); kompleks, plazmid DNA yarılması, kısmi interkalasyon, elektrostatik etkileşim Synthesis and Investigation of DNA Effect of [N,N`-bis insert ignore into journalissuearticles values(salicylidene);-1,3-propanediaminato]nickelinsert ignore into journalissuearticles values(II); Complex Abstract: In this study, N,N`-bisinsert ignore into journalissuearticles values(salicylidene);-1,3-propanediaminato]nickelinsert ignore into journalissuearticles values(II); insert ignore into journalissuearticles values(NiL); complex was synthesized from reaction of bisinsert ignore into journalissuearticles values(salicylidene);-1,3-propanediamine insert ignore into journalissuearticles values(H2L); with nickelinsert ignore into journalissuearticles values(II); chloride hexahydrate in ethanolic medium. The interaction of synthesized NiL complex with pBR322 plasmid DNA was investigated by ultraviolet absorption spectroscopy insert ignore into journalissuearticles values(UV);, cyclic voltammetry insert ignore into journalissuearticles values(CV); and agarose jel electrophoresis. It was found that the absorption spectrum of NiL complex in the presence of plasmid DNA showed hyperchromism and its peak current was significantly decreased at recorded voltammogram. Effect of NiL complex on DNA in the absence of reductant agents showed by ajarose jel electrophoresis. Moreover, the geometry of the lowest-energy structure of NiL complex calculated by Hyperchem® program gave a good result to be associated with partial intercalation and electrostatic binding modes of the DNA. Key words: Niinsert ignore into journalissuearticles values(II); complex, plasmid DNA cleavage, partial intercalation, electrostatic interaction
Keywords : Nikel II, kompleks, plazmid DNA yarılması, kısmi interkalasyon, elektrostatik etkileşim