Polymorphisms of TLR1, TLR4 and SLC11A1 Genes in Some Cattle Breeds Reared in Turkey

Authors : Korhan ARSLAN
Pages : 547-553
Doi:10.15832/ankutbd.349728
View : 43 | Download : 16
Publication Date : 2018-12-05
Article Type : Research Paper
Abstract :The objective of this study was to examine the allelic and genotypic profiles of four SNPs of bovine Toll-Like receptor 1 insert ignore into journalissuearticles values(TLR1);, Toll-Like Receptor 4 insert ignore into journalissuearticles values(TLR4); and Solute Linkete Carier 11A1 insert ignore into journalissuearticles values(SLC11A1); genes in Holstein, two Turkish native breeds and their crossbreds in Turkey. For this purpose, a total of 1023 cattle from Holstein insert ignore into journalissuearticles values(HL, n= 410);, Anatolian Black insert ignore into journalissuearticles values(AB, n= 106);, East Anatolian Red insert ignore into journalissuearticles values(EAR, n= 84);, Anatolian Black Crossbreed insert ignore into journalissuearticles values(ABC, n= 124); and East Anatolian Red crossbreed insert ignore into journalissuearticles values(EARC, n= 299); breeds were examined for four SNPs. Samples were genotyped using by the PCR-RFLP method. According to the TLR1 insert ignore into journalissuearticles values(+1380); SNP, EAR was in Hardy-Weinberg equilibrium insert ignore into journalissuearticles values(HWE); while the AB, HL and native crossbreeds were deviated from HWE. All cattle breeds were in HWE for the TLR1 insert ignore into journalissuearticles values(+1596); SNP. In terms of TLR4 insert ignore into journalissuearticles values(+10); SNP, the CC genotype had the highest frequency in HL and native crossbreeds whereas the AB and EAR breeds were monomorphic and only CC genotype was found these breeds. According to the SLC11A1 insert ignore into journalissuearticles values(+1066); SNP, the CC genotype had the highest while the GG genotype frequency the lowest in all breeds. In addition EAR breed was in HWE while the AB, HL and native crossbreeds deviated from HWE.
Keywords : Polymorphism, Turkish native cattle, Toll like receptor, SLC11A1, Marker assisted selection

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