Increasing of Phenolic Compounds by Brassinosteroid Applications in Immobilized Cell Suspension Cultures of Vitis vinifera L. cv. Cinsault

Authors : Zehra BABALİK

Pages : 298-303

Doi:10.15832/ankutbd.674860

View : 49 | Download : 18

Publication Date : 2021-09-04

Article Type : Research Paper

Abstract :In this paper, the effects on secondary metabolite accumulation of brassinosteroid insert ignore into journalissuearticles values(BR); insert ignore into journalissuearticles values(24-epibrassinolide insert ignore into journalissuearticles values(24-eBL); on immobilized cells that were obtained from Vitis vinifera cv. Cinsault was investigated. 24-eBL was treated to immobilized cells covered calcium alginate beads at concentrations of 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 for one month. As a result of this study, it was found that 24-eBL applications modified secondary metabolite accumulation and had positive effects on secondary metabolite production when the suitable concentration was used. While the highest total phenolic, catechin, p-coumaric acid and chlorogenic acid contents were found in immobilized cells treated 0.75 mg L-1 24-eBL, the highest epicatechin, quercetin, trans-resveratrol contents were obtained in immobilized cells treated 0.50 mg L-1 24-eBL and the highest gallic acid content was determined in immobilized cells treated 0.25 mg L-1 24-eBL. Moreover, the highest 24-eBL concentration insert ignore into journalissuearticles values(1 mg L-1); decreased the content of secondary metabolite compared to the control insert ignore into journalissuearticles values(0 mg L-1 24-eBL); except total phenolic and catechin content. To conclude, 0.50 and 0.75 mg L-1 24-eBL concentrations were the most suitable concentrations for immobilized cell culture to provide the highest secondary metabolite accumulation.
Keywords : 24 eBL, immobilization, grapevine, phenolic compounds

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