Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources using Allele-specific PCR

Authors : Merve Dilek KARATAŞ, Nahid HAZRATİ, Canan YÜKSEL ÖZMEN, Mohammad HASANZADEH, Serdar ALTINTAŞ, Mehmet Emin AKÇAY, Ali ERGÜL

Pages : 287-297

Doi:10.15832/ankutbd.930238

View : 48 | Download : 10

Publication Date : 2023-01-31

Article Type : Research Paper

Abstract :Self-incompatibility insert ignore into journalissuearticles values(SI); is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple insert ignore into journalissuearticles values(Malus domestica L.);, self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes insert ignore into journalissuearticles values(such as, Amasya, Hüryemez, Şah elması, Tokat, Demir elması etc.); obtained from the National Collection of Atatürk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR insert ignore into journalissuearticles values(Polymerase Chain Reaction); using 4 different S-alleles insert ignore into journalissuearticles values(Sd, Sf, S26 and S9);. 181 genotypes containing at least 1 S-allele were identified as ‘Partially Incompatible’ and 12 genotypes involving 4 S-alleles were assigned ‘Totally Incompatible’. No S-alleles were observed in 2 genotypes insert ignore into journalissuearticles values(Pancarlık and Hüryemez); which exhibited ‘Compatibility’ status.
Keywords : Malus domestica L, Self incompatibility, Anatolian gen resourses, S alleles

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