Effects of Dietary Sage, Myrtle and/or Probiotic Mixture on Growth, Intestinal Health, Antioxidant Capacity, and Diseases Resistance of Oncorhynchus mykiss

Authors : Öznur ÖZİL, Öznur DİLER, Muhammet Hayati KAYHAN, Tugba KÖK TAŞ, Zeynep SEYDİM, Behire IŞIL DİDİNEN

Pages : 721-733

Doi:10.15832/ankutbd.1120481

View : 44 | Download : 11

Publication Date : 2023-03-31

Article Type : Research Paper

Abstract :It is widely known that the use of medicinal plants and probiotics as feed additives has a positive effect on growth, non-specific immune system, and resistance to diseases in aquaculture. This study examines the effects of dietary supplementation with sage insert ignore into journalissuearticles values(Salvia officinalis); and myrtle insert ignore into journalissuearticles values(Myrtus communis);, alone or in combination with a probiotic mixture insert ignore into journalissuearticles values(PM); on growth, intestine microflora and histology, some antioxidant enzymes activities in the muscle tissues of rainbow trout insert ignore into journalissuearticles values(Oncorhynchus mykiss); and disease resistance against Vibrio anguillarum. For this purpose, fish were fed with a control diet of 1% sage, 1% myrtle, 1.1% probiotic mixture, 1% sage +1.1% PM and 1% myrtle+1.1% PM supplemented diets for 60 days. At the end of the trial, the fish fed the diets supplemented with myrtle and sage + PM showed a positive effect on feed conversion ratio. According to the histological assessment, the villi length, villi width and goblet cell numbers in the intestines of fish in all groups increased compared to the control. Superoxide dismutase activity in the muscles of fish in the PM group was higher than the fish in the other groups insert ignore into journalissuearticles values(p<0.05);. The malondialdehyde activity was unaffected with the exception of the fish in the sage group insert ignore into journalissuearticles values(p<0.05);. The lactic acid bacteria count in the intestines increased in fish fed the sage + PM insert ignore into journalissuearticles values(p<0.05);. Fish fed the diets supplemented with sage + probiotic mixture, probiotic mixture, myrtle + probiotic mixture, and myrtle saw a significant reduction in mortality insert ignore into journalissuearticles values(0-32.5%); due to V. anguillarum compared to the control insert ignore into journalissuearticles values(63.2%); insert ignore into journalissuearticles values(p<0.05);. In conclusion, the use of probiotics, sage and myrtle in combination as a feed supplement showed a positive effect on the growth performance, intestinal microflora and histology, and antioxidant enzymes activities and disease resistance in rainbow trout.
Keywords : Salvia officinalis, Myrtus communis, Probiotic, Histology, Vibrio anguillarum, Rainbow trout

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