Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti

Authors : Özer AKGÜL, Hayriye KIRKOYUN UYSAL, İlker TOSUN, Burcu SAPMAZ, Reyhan ÇALIŞKAN, A Canan YAZICI GÜVERCİN, Sinem ÖKTEM, Özgür KURT, Y Ali ÖNER

Pages : 17-23

View : 26 | Download : 5

Publication Date : 2019-01-31

Article Type : Research Paper

Abstract :Objective: Intestinal parasitic infections are among the prominent public health concerns in patients with suppressed immune function. Material and methods: Here we examined stool specimens by microscopy and polymerase chain reaction insert ignore into journalissuearticles values(PCR); to evaluate the infection of five principal protozoans insert ignore into journalissuearticles values( Cryptosporidium spp., Giardia spp., Entamoeba histolytica , Blastocystis spp. and Dientamoeba spp.); among 90 renal transplant recipient patients insert ignore into journalissuearticles values(RTP); in comparison with 90 healthy individuals insert ignore into journalissuearticles values(HI); from Turkey. Results: The overall frequency of any parasites was 17.2% insert ignore into journalissuearticles values(31/180); with microscopy and 51.7% insert ignore into journalissuearticles values(93/180); with PCR. Because of its high sensitivity, PCR was compared with microscopy in terms of the accuracy of detecting intestinal parasites, and the agreement was found to be inadequate insert ignore into journalissuearticles values(κ= 0.217; p<0.001);. Multiparasitism insert ignore into journalissuearticles values(90.9%);, Cryptosporidium spp. insert ignore into journalissuearticles values(84.6%); and Giardia spp. insert ignore into journalissuearticles values(74.1%); were the most frequent agents in RTP, respectively insert ignore into journalissuearticles values(p<0.001);. Conclusions: This is the first study performed in Turkish reporting the prevalence of five intestinal parasites with PCR techniques among this group and seeks to provide a basis for future studies   
Keywords : Intestinal parasites, Transplantation, PCR, Microscopy