Determination of TYLCV-Resistant Cherry and Cocktail Tomato Cultivars by Molecular Markers

Authors : Hüseyin Basim, Osman Kandil, Mine Karaoğlan

Pages : 89-96

Doi:10.55979/tjse.1357477

View : 47 | Download : 67

Publication Date : 2023-12-29

Article Type : Research Paper

Abstract :ABSTRACT In this study, resistant cultivars to Tomato yellow leaf curly virus (TYLCV) of different cherry and cocktail tomato cultivars were determined using molecular DNA markers. For this purpose, resistance to Tomato Yellow Leaf Curly Virus (TYLCV) of a total of 409 different cherry and cocktail tomato cultivars was determined by polymerase chain reaction (PCR) using the primer Ty3P6-25. As a result of the assays, 291 cherry and cocktail tomato cultivars were found to be susceptible (rr), 66 cultivars were heterozygous resistant (Rr), and 45 cultivars were homozygous resistant (RR) to TYLCV. In addition, no molecular markers were detected in 7 cherry and cocktail tomato cultivars. It was found that the molecular DNA marker used is useful in determining resistance responses to TYLCV in cherry tomato and cocktail tomato and can provide reproducible and reliable results in a short time.
Keywords : Domates Sarı Yaprak Kıvrılma Virüsü, Kiraz ve kokteyl domatesleri, Moleküler markör