Purification and Charcterızatıon Glutathione S-Transferase Enzyme From Chicken Liver

Authors : Hakan YILMAZ, Mehmet ÇİFTCİ, Yusuf TEMEL

Pages : 117-124

Doi:10.46810/tdfd.1242764

View : 76 | Download : 9

Publication Date : 2023-03-27

Article Type : Research Paper

Abstract :In this study, the glutathione S-transferase enzyme insert ignore into journalissuearticles values(GST; EC 2.5.1.18); was purified with 8.35 EU/mL specific activity, 24.56 times 8% yield, from chicken liver, using ammonium sulfate precipitation and glutathione-agarose affinity chromatography. In order to control the purity of the enzyme, SDS-PAGE was performed and a single band was obtained. The molecular mass of the subunit was calculated as approximately 30.9 kDa. In addition, the optimum pH value of the enzyme insert ignore into journalissuearticles values(8.5 in Tris-HCl);; optimum ionic strength insert ignore into journalissuearticles values(150 mM with Tris-HCl);; optimum temperature insert ignore into journalissuearticles values(70 oC);; stable pH value insert ignore into journalissuearticles values(8.5 with Tris-HCl); was determined. The KM value for the GSH substrate of the enzyme was 0.802 mM, the Vmax value was 1.833 EU/mL; For CDNB, the KM value was calculated as 3.6 mM and the Vmax value was calculated as 2.829 EU/mL.
Keywords : Glutatyon S transferaz, tavuk karaciğeri, saflaştırma, Glutathione S transferase, chicken liver, purification

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