Phytochemical contents and in vitro activities of Berberis thunbergii hydroethanolic extract

Authors : Elif Ceren Karakuş, Tevfik Ozen, Semiha Yenigün, Yunus Başar, İbrahim Demirtas, İlyas Yıldız

Pages : 183-191

Doi:10.46810/tdfd.1664372

View : 81 | Download : 114

Publication Date : 2025-09-26

Article Type : Research Paper

Abstract :Berberis thunbergii, also known as Japanese dogwood, has many medicinal properties due to its rich phytochemical components. Therefore, B. thunbergii hydroethanolic extract was investigated phytochemical content, antioxidant, anti-inflammatory, and enzyme inhibition activities. In LC-MS/MS and GC-MS/MS analysis, the main components of the extract were determined as chlorogenic and oleic acid, respectively. Total phenol and flavonoid amounts were found as 259.40±0.96 mg GAE/g extract and 2.60±0.57 mg QE/g extract, respectively. It was recorded that it could reduce iron as 6.38 mg TE/g extract in the FRAP test and had a radical scavenging effect of 85.26% in the DPPH֗ scavenging test. The anti-inflammatory property of the extract was found to be 60.80±2.22 µg/mL, while DFS was found to be 45.23±0.68. Additionally, in enzyme inhibition, it was observed that it inhibited urease at a low level of 44.19±0.22 µg/mL and xanthine oxidase at a high level of 0.91±0.21 µg/mL. It was recorded that it protected DNA with a 57.55% Form I value. Due to its high antioxidant and XO inhibition effects, B. thunbergii is thought to have potential applications in food, cosmetics, and medicine.
Keywords : Berberis thunbergii, Hydroethanolic extract, Phytochemical, In vitro bioactivity

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