The Protective Effect of Hydralazine against Hydrogen Peroxide (H2O2)-Induced Oxidative Damage in C6 Glial Cell Line

Authors : Ahmet Şevki TAŞKIRAN, Mustafa ERGÜL

Pages : 8-15

View : 17 | Download : 10

Publication Date : 2021-01-29

Article Type : Research Paper

Abstract :Purpose: Recent studies have shown that hydralazine has positive effects on nervous system. However, its effect on hydrogen peroxide-induced oxidative damage in glial cells is still unclear. The current experiment was designed to examine the effect of hydralazine on glial damage after hydrogen peroxide-induced oxidative damage in C6 glial cells involving proinflammatory cytokines. Material and Methods: In this study, the C6 glioma cell line was used. Four cell groups were prepared to evaluate the effect of hydralazine on glial cell death after hydrogen peroxide-induced oxidative damage. The control group was without any treatment. Cells in the H2O2 group were treated with 0.5 mM H2O2 for 24 hours. Cells in the hydralazine group were treated with various concentrations insert ignore into journalissuearticles values(12.5, 25, 50, and 100 µM/mL); of hydralazine for 24 hours. Cells in the hydralazine + H2O2 group were pre-treated with various concentrations insert ignore into journalissuearticles values(12.5, 25 50, and 100 µM/mL); of hydralazine for 1 hour and then exposed to 0.5 mM H2O2 for 24 hours. After completing oxidative damage induction, the cell viability was evaluated XTT assay. Total antioxidant status insert ignore into journalissuearticles values(TAS);, total oxidant status insert ignore into journalissuearticles values(TOS);, tumor necrosis factor alpha insert ignore into journalissuearticles values(TNF-α);, and interleukin-1 beta insert ignore into journalissuearticles values(IL-1 β); levels in the cells were measured by commercial kits. Results: Hydralazine at the concentrations of 50 and 100 µM/mL significantly increased the cell viability in C6 cells after hydrogen peroxide-induced oxidative damage insert ignore into journalissuearticles values(p < 0.001);. It also significantly decreased the levels of TOS insert ignore into journalissuearticles values(p < 0.001); whereas rising TAS levels insert ignore into journalissuearticles values(p < 0.01); after hydrogen peroxide-induced oxidative damage. Moreover, hydralazine reduced TNF-α and IL-1 β levels in C6 cells after hydrogen peroxide-induced oxidative damage insert ignore into journalissuearticles values(P < 0.001);. Conclusion: Hydralazine decreases glial cell death after hydrogen peroxide-induced oxidative damage in C6 cells by activating antioxidant system and inhibiting proinflammatory cytokines.
Keywords : Hydralazine, Oxidative Stress, Cell Death, Proinflammatory Cytokines, C6 Glioma