Influence of Zebularin on Antioxidant Enzymes and Lipid Peroxidation of Galleria mellonella (Lepidoptera: Pyralidae)

Authors : Ebru ÇAKIR, Fevzi UÇKAN, Ekrem ERGİN, Rabia ÖZBEK

Pages : 457-464

Doi:10.30910/turkjans.1110745

View : 17 | Download : 13

Publication Date : 2023-04-25

Article Type : Research Paper

Abstract :Cytosine methylation is a process that adds a methyl group to a cytosine residue of DNA to convert it to 5-methylcytosine. Zebularine insert ignore into journalissuearticles values(ZEB); reactivates hypermethylated genes in yeast and solid tumor cells, with DNA methyltransferases that show a preferential effect on cancer cells. We investigated the effects of various doses insert ignore into journalissuearticles values(0.25-32 mg/ml); of ZEB, on antioxidant enzyme activity and lipid peroxidation in wax moth Galleria mellonella last instars. Injection of ZEB in last instars G. mellonella resulted in increases in SOD, MDA, and GST levels, in order of magnitude, but not in CAT enzyme activity. Two possible reasons were predicted for these results. This effect could be a result of the inhibitory effect of ZEB on DNA methylation in G. mellonella, which leaded to reducing gene transcription in insects. Or it was likely that high oxidative stress might cause a decreasing activity of enzymes.
Keywords : antioksidan enzimleri, DNA metilasyonu, Galleria mellonella, zebularin, Lipit peroksidasyonu