Insecticide resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields in Çukurova Region (Türkiye)

Authors : Selçuk Ulusoy, Doğancan Kahya, Mustafa Gökhan, Okan Özgür

Pages : 387-399

Doi:10.16970/entoted.1344982

View : 199 | Download : 291

Publication Date : 2023-12-01

Article Type : Research Paper

Abstract :This study aimed to reveal resistance levels of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) populations against dimethoate, λ-cyhalothrin and chlorpyrifos-ethyl used in cotton fields in Adana province in Çukurova Region (Türkiye). Bioassay, biochemical and molecular methods were used to determine resistance on the populations collected from 16 locations between 2020 and 2021. Six populations were resistant according to the susceptible Toktamış population with leaf dip discriminating dose bioassays. Compared to the susceptible population, four populations were found at decreased susceptibility (DS) resistance levels to dimethoate and one population to chlorpyrifos-ethyl. Only two populations resistance ratio were detected in MR (Moderate resistance) category to chlorpyrifos-ethyl. Resistance levels of other populations were observed as S (susceptible) category. Resistant populations had higher acetylcholinesterase, glutathione-S transferase and cytochrome P450 monooxygenase enzyme activities in biochemical analysis. The carboxylesterase gene transcription levels were higher in resistant populations. S431F and Kdr (knockdown) mutation were determined by the PCR-RLFP method, which is effective in organophosphate and pyrethroid insecticides resistance and 17% and 100% recessive alleles were detected in populations. The biochemical and mutation-induced resistance to dimethoate and chlorpyrifos-ethyl was detected. These results will contribute to developing strategies for resistance management of A. gossypii.
Keywords : Aphis gossypii, direnç, insektisit, Türkiye, organikfosfatlılar, piretroit