Antioxidant Activities and Enzyme Inhibition Potentials of Hypericum perforatum L. Ethanol Extracts

Authors : Melek ÇOL AYVAZ, Gülizar AYDOĞDU, Zeynep KOLOREN, Onur KOLÖREN, Panagiotis KARANİS

Pages : 49-57

View : 60 | Download : 72

Publication Date : 2023-07-10

Article Type : Research Paper

Abstract :This study aimed to investigate the antioxidant activities, enzyme inhibition potentials, total phenolic and flavonoid contents of Hypericum perforatum L. ethanol extracts. The phenolic content of the plant extract was determined using the Folin-Ciocalteu reagent. The antioxidant activities of the plant extract were determined based on the iron-reducing power and scavenging potentials of the DPPH radical. Furthermore, chelating ability, superoxide dismutase and in vitro anti-inflammatory activity, tyrosinase α-glucosidase, and cholinesterase inhibition assays were determined by spectrophotometric techniques. The plant extract\`s total phenolic and flavonoid contents were 8.828 mg GAE/g and 10.366 mg CTE/g extract, respectively. H. perforatum ethanolic extract exhibited scavenging of 1,1-Diphenyl-2-Picrylhydrazyl insert ignore into journalissuearticles values(DPPH); with an SC50 value of 0.469 mg/mL and superoxide anion radicals with IC50 values of 0.338 mg/mL. Ferric-ion chelating insert ignore into journalissuearticles values(%4.7 in 0.5 mg/mL);, anti-inflammatory activity insert ignore into journalissuearticles values(%25.12 in 0.2 mg/mL);, acetylcholinesterase inhibition insert ignore into journalissuearticles values(%18 in 0.2 mg/mL);, butyrylcholinesterase inhibition insert ignore into journalissuearticles values(%15 in 0.2 mg/mL);, tyrosinase inhibition insert ignore into journalissuearticles values(%35 in 0.5 mg/mL);, and α-glucosidase inhibition insert ignore into journalissuearticles values(%35 in 0.2 mg/mL); activities of the plant extract were determined.
Keywords : Antioxidant activity, Ferric ion chelating, anti inflammatory activity, enzyme inhibition potentials