Investigation of salivary miRNA-199a-5p levels in plaque-induced gingivitis

Authors : Fatma Tuba Akdeniz, Ahmet Mert Nalbantoğlu, Zerrin Barut, Şule Bulut, Orçun Avşar, Hilal Korkmaz Karadeniz, Turgay İsbir

Pages : 369-374

Doi:10.38053/acmj.1676243

View : 50 | Download : 80

Publication Date : 2025-05-30

Article Type : Research Paper

Abstract :Aims: Epigenetic mechanisms play a crucial role in regulating the response to chronic inflammation in periodontal diseases and serve as reliable biomarkers. While these biomarkers are traditionally assessed through invasive techniques, analyzing miRNA expression in saliva offers a non-invasive and convenient alternative. Our study aims to investigate salivary miRNA-199a-5p levels in both healthy and diseased conditions and to evaluate its potential role as an early diagnostic indicator of periodontal diseases such as gingivitis. Methods: Saliva samples from 50 individuals, either diagnosed with gingivitis or exhibiting a healthy periodontal condition, were analyzed using miRNeasy serum/plasma kit (Qiagen). SPSS software was used for statistical analysis. MiRNA-199a-5p expression levels were compared using the Mann-Whitney U test. Results: In saliva samples collected from 50 individuals, the expression levels of miRNA-199a-5p were significantly elevated in the gingivitis group compared to the healthy control group. Aditionally, the bleeding on probing (BOP %) values were significantly higher in group G compared to the healthy group. Conclusion: miRNA-199a-5p can be considered an important regulator with potential as both an early diagnostic biomarker and a therapeutic target for periodontal diseases. Although its role in autophagy and inflammation shows promise, further studies are needed to clarify its specific functions and clinical relevance.
Keywords : miRNA-199a-5p, gingivitis, periodontal disease

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