Investigation of Drug Resistant Mycobacterium tuberculosis Strains with Molecular Methods

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Uludağ Üniversitesi Tıp Fakültesi Dergisi
Cilt: 51 Sayı: 2
Investigation of Drug Resistant Mycobacterium tuberculosis Strains with Molecular Methods

Investigation of Drug Resistant Mycobacterium tuberculosis Strains with Molecular Methods

Authors : Ayşe Melda Payaslıoğlu, Sezcan Sağlam, Seçil Ak Aksoy, Cüneyt Özakın

Pages : 291-294

Doi:10.32708/uutfd.1723791

View : 37 | Download : 36

Publication Date : 2025-08-28

Article Type : Research Paper

Abstract :The aim of this study was to investigate mutations in the rpoB and embB genes using molecular methods in Mycobacterium tuberculosis isolates phenotypically resistant to rifampicin and/or ethambutol. Drug susceptibility testing of 110 isolates collected in Turkey between 2005 and 2014 was performed using the modified agar proportion method. Molecular analysis was conducted on 28 selected isolates, including 7 rifampicin-resistant strains. Mutations were detected within the Rifampicin Resistance-Determining Region (RRDR) between codons 507–533 of the rpoB gene. The most common mutation was Ser531Leu (TCG→TTG), found in 10 strains; one strain had a His526Asn (CAC→AAC) mutation. These mutations were considered important molecular markers of rifampicin resistance. Rapid molecular detection of these mutations is important for early and effective tuberculosis treatment planning.
Keywords : mycobacterium tuberculosis, drug resistance, moleküler analiz

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