Determination of the effect of green extraction solvents on the phenolic acids and flavonoids of propolis

Authors : Meral KEKEÇOĞLU, Ali SORUCU

Pages : 49-54

Doi:10.30782/jrvm.937418

View : 23 | Download : 11

Publication Date : 2022-07-26

Article Type : Research Paper

Abstract :The present study investigated the extraction of phenolic compounds from propolis according to solvent factor. The propolis samples were extracted in four different solvents, which water, ethanol-water insert ignore into journalissuearticles values(70%);, dimethyl sulfoxide insert ignore into journalissuearticles values(DMSO);, and L-lysine insert ignore into journalissuearticles values(8%);, and were analyzed 36 phenolic compounds by HPLC-DAD. Statistically significant differences in solubility of the phenolic compound at various levels were detected among the solvents insert ignore into journalissuearticles values(P <0.05);. Only water and ethanol-water insert ignore into journalissuearticles values(70%); were determined more successful than the other solvents in the extraction of phenolic components of propolis. Phenolic acids generally dissolved higher in water, while flavonoids dissolved higher in ethanol were determined. Certain phenolic compounds were detected only in some of the propolis extract which are syringic acid and daidzein in water, vitexin, rutin, and epigallocatechin in ethanol, and emodin in DMSO. Consequently, the chemical content affected significantly depending on the extraction solvent of propolis. Therefore, it is important to determine the extraction solvent and analyses of propolis before application for therapeutic purposes.
Keywords : Propolis extraction, Water, Ethanol, DMSO, L lysine, Phenolic compound