Production Of Hand Sterilizatıon Fluid Of Herbal Origin

Authors : Sergen CETEBOZAN, Hatice Kubra OZGUL, Sibel YİGİTARSLAN

Pages : 175-184

View : 44 | Download : 14

Publication Date : 2019-12-16

Article Type : Research Paper

Abstract :When the body is exposed to microorganisms of daily life, their negative effects on human health will be observed. Industrial disinfection gels especially used for effective and rapid hand disinfection, contain ethyl or isopropyl alcohol. Due to the presence of alcohol, they cause some allergic reactions on the skin. The aim of this study was to investigate the usability of flavonoids obtained from horse chestnut shells as hand disinfection gel material. Single and multi-parameter optimizations were applied to obtain the maximum amount of flavonoids from the material by ultrasonic extraction. In single optimization, it was determined that application of 50 o C, 2g-plant/100ml-water, 10 min-continuous sound waves increased the extraction efficiency. To determine the optimum values ​​of parameters in multiple optimization, Response-Surface Method including Box-Benken design was used via Design-Expert program. As a result of the numerical solution, application of ultrasonic extraction to 2.44g of horse chestnut and 100ml of water at 40.1 o C for 14.89 minutes produced the highest flavonoid content. The amount of antioxidant in extracts was analyzed spectrophotometrically by DPPH method. Addition of 9.6ml of the extract to mixed microorganism culture media resulted successful inhibition. Thus, it was concluded that the disinfection liquid containg the same concentration produced by thickening it with sodium arginate was found as an alternative to the existing product containing synthetic chemicals.
Keywords : Aesculus hippocastanum, DPPH, Ultrasonic extraction

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