Immunohistochemical and Stereological Examination of The Gastrocnemius Muscle In Rats Applied with Botox

Authors : Mehmet Uğur Delibaş, Gamze Çakmak

Pages : 166-174

Doi:10.52976/vansaglik.1552892

View : 53 | Download : 54

Publication Date : 2024-12-30

Article Type : Research Paper

Abstract :The aim of this study was to examine immunohistochemically and stereologically the volume density of male rats to which botox was applied to the gastrocnemius sinistra muscle, on the 15th and 21st days. 30 Wistar Albino rats, 3 different three-month-old groups, were used. Botox A solution, 2.5U in 0.1ml physiological saline, was prepared and injected into the gastrocnemius sinistra muscle of the rats in the other two groups except the control group. Rats were fixed by perfusion with 10% buffered formaldehyde. Musculus gastrocnemius sinistra of the rats were dissected one week after the perfusion process. Sections were randomly selected among the first 15 sections, and each subsequent 80th section was taken by systematic random sampling method. Sections were obtained with a thickness of 5µm and 8-10 sections. The sections were stained with hematoxylin and eosin, and photographed under a microscope. Total volume values of musculus gasrocnemius sinistra were determined in accordance with the Cavalieri’s Principle and using the Shtereom I package program. It was determined that the volume values between the groups showed a statistically significant difference (p<0.05). In addition, the effects of Botox A on the gastrocnemius muscle on the 15th and 21st days were detected immunohistochemically using Caspase 3 antibody dye.
Keywords : Botox, Hacim, İmmunohistokimyal, Musculus gastrocnemius, Stereoloji.

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