COMPARISON OF SOME BIOLOGICAL ACTIVITIES AND CATECHIN TANNIN CONTENTS OF TWO JUNIPERUS AND PRUNUS SPECIES

Authors : Hafize YUCA, Hakkı Cem DEMİRCAN, Bilge AYDIN, Mehmet ÖNAL, Enes TEKMAN, Ayşe CİVAŞ, Mohaddeseh NOBARİREZAEYEH, Gamze GÖGER, Songül KARAKAYA, Zuhal GÜVENALP

Pages : 650-666

Doi:10.33483/jfpau.1218845

View : 20 | Download : 13

Publication Date : 2023-05-20

Article Type : Research Paper

Abstract :Objective: Qualitative and quantitative determination of catechin in fruits of J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, and P. cerasifera was analyzed by LC-MS/MS using standards. Antidiabetic, antimicrobial, and antioxidant activities of fruit and cone extracts of these plants were evaluated. Qualitative analysis of secondary metabolites was also done. Material and Method: Antimicrobial activity was done with MIC. ABTS·+ and DPPH• scavenging activies were used antioxidant activity. α-Amylase and α-glucosidase inhibition assays were done for antidiabetic activity. Result and Discussion: JCS insert ignore into journalissuearticles values(IC50 = 578 ± 20 μg/ml);, JOO insert ignore into journalissuearticles values(IC50 = 3706 ± 52 μg/ml);, and PS insert ignore into journalissuearticles values(IC50 = 313 ± 7 μg/ml); extracts were observed to show a higher activity than acarbose insert ignore into journalissuearticles values(IC50 = 4061 ± 146 μg/ml);. J. communis var. saxatilis fruit extract was observed with MIC values between insert ignore into journalissuearticles values(312.5 -2500 µg/ml); against all microorganisms. JCS extract has the highest phenolic composition and JOO has the lowest phenolic composition insert ignore into journalissuearticles values(µg GAE/ mg extract);. JCS extract indicated the highest antioxidant activity. It was determined that plant containing the highest catechin insert ignore into journalissuearticles values(1173.3±5.77 ng/ml); and epigallocatechin insert ignore into journalissuearticles values(208±7.21 ng/ml); was JCS. In this research, it can be said that JCS with the highest tannin content shows the highest effects.
Keywords : Antidiyabetik, antimikrobiyal, kateşik, Juniperus, Prunus