NEPHROPROTECTIVE EFFECT OF ALOE VERA EXTRACT WITH REGULATION OF OXIDATIVE STRESS, APOPTOSIS AND AQUAPORIN 3 EXPRESSION LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Authors : Uğur ŞEKER, Barış Can GÜZEL, Dila ŞENER, Saime Betül BAYGELDİ, Meral YÜKSEL, Özlem UNAY DEMİREL, Sevda SOKER

Pages : 438-449

Doi:10.33483/jfpau.1225760

View : 22 | Download : 14

Publication Date : 2023-05-20

Article Type : Research Paper

Abstract :Objective: In this study we examined the protective activity of Aloe vera with considering anti-oxidant, anti-apoptotic properties, and the status of Aquaporin 3 insert ignore into journalissuearticles values(AQP3); channel protein. Material and Method: Twenty-one adult female rats were divided into three groups insert ignore into journalissuearticles values(n=7);; Control, Diabetes, Treatment. Control group did not expose to any application. Animals in Diabetes and Treatment were exposed to experimental diabetes with administration of streptozotocin. Rats in Treatment received 300 mg/kg Aloe vera extract daily for 14 days. Rats were sacrificed and kidney samples were used for analyses. Result and Discussion: Analyses indicated that lowest malondialdehyde insert ignore into journalissuearticles values(MDA); and luminol levels in control group were increased significantly insert ignore into journalissuearticles values(P<0.05); in diabetic animals. Severe pathological changes observed in Diabetes group while microscopic examinations. Bax, Caspase-3 and apoptotic index insert ignore into journalissuearticles values(AI); were elevated significantly insert ignore into journalissuearticles values(P<0.05); in this group compared to Control. Oxidative stress, apoptotic protein expression levels and TUNEL Assay positive cell ratio were down-regulated in Treatment group. When AQP3 levels were measured, immunopositivity reduced significantly insert ignore into journalissuearticles values(P<0.05); in cortical kidney of Diabetes group which is normalized significantly in Treatment group.This study reporting anti-diabetic potency of Aloe vera extract has capability to avoid streptozotocin induced diabetic renal injury via regulating anti-apoptotic and anti-oxidant cellular signaling. Furthermore, Aloe vera consumption in diabetes might regulate AQP3 levels. Although we observed promising results, more studies are required to explore anti-diabetic, anti-hyperglycemic and nephroprotective activity of Aloe vera.
Keywords : Aloe vera, apoptoz, aquaporin 3, böbrek, diyabet