ANTIANGIOGENIC ACTIVITY AND ROS-MEDIATED LUNG CANCER CELL LINE INJURY OF ZERUMBONE

Authors : Shaymaa Fadhel ABBAS ALBAAYİT, Maryam Kamel MOHAMMED

Pages : 731-738

Doi:10.33483/jfpau.1112778

View : 104 | Download : 73

Publication Date : 2023-09-20

Article Type : Research Paper

Abstract :Objective: Zerumbone insert ignore into journalissuearticles values(ZER); is a well-known natural compound that has been reported to have anti-cancer effect. Thus, this study investigated the ZER potential to inhibit Thymidine Phosphorylase insert ignore into journalissuearticles values(TP); and the ability to trigger Reactive oxygen species insert ignore into journalissuearticles values(ROS);-mediated cytotoxicity in non-small cell lung cancer, NCI-H460, cell line. Material and Method: The antiangiogenic activity for ZER was evaluated by using the thymidine phosphorylase inhibitory test. Reactive oxygen species insert ignore into journalissuearticles values(ROS); production was determined via DCFDA dye by using flow cytometry. Result and Discussion: ZER was found to be potent TP inhibitory with the IC50 value of 50.3± 0.31 μg/ml or 230±1.42 µM. NCI-H460 cells upon treatment with ZER produced significant ROS by 55.7%. Consequently, ZER exerts anti-angiogenic properties and modulates ROS production in lung cancer cells, serving as leads for better therapeutic index in cancer drug.
Keywords : Akış sitometrisi, NCI H460, timidin fosforilaz

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