DETERMINATION OF DNA DAMAGE INDUCED BY BISPHENOL A AND BISPHENOL S IN MCF7 CELL LINE

Authors : Ekin Erdoğmuş, Seda Ipek Tekneci, Belma Gümüşel, Yalçın Duydu, Aylin Üstündağ

Pages : 552-558

Doi:10.33483/jfpau.1428739

View : 53 | Download : 83

Publication Date : 2024-05-20

Article Type : Research Paper

Abstract :Objective: This study aimed to determine the DNA damage induced by Bisphenol A (BPA) and Bisphenol S (BPS) on MCF7 cell line. Material and Method: DNA damage was determined by COMET assay in MCF7 cell line at 0.1, 0.5, 1, 5, 10 and 50 μM concentrations of BPA and BPS. Result and Discussion: All BPA and BPS concentrations studied (0.1, 0.5, 1, 5, 10 and 50 μM) significantly induced DNA damage on MCF7 cell line compared with control (p<0.05). BPS significantly induced DNA damage more than BPA at the 3 highest concentrations studied (5, 10 and 50 μM) (p<0.05). This study shows that bisphenol derivatives can also cause DNA damage like BPA.
Keywords : Bisfenol A, bisfenol S, COMET testi, DNA hasarı, MCF7 hücre hattı