INVESTIGATION OF BIOCHEMICAL PROPERTIES, ANTIMICROBIAL, ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF THE RHAMNUS CATHARTICA L. EXTRACTS COLLECTED FROM KAZAKHSTAN

Authors : Sofiakhan Alimova, Yessimsiitova Zura, Tutku Tunç, Hülya Özpınar
Pages : 743-755
Doi:10.33483/jfpau.1595627
View : 80 | Download : 63
Publication Date : 2025-09-19
Article Type : Research Paper
Abstract :Objectives: This research evaluated the biochemical composition of hexane, ethyl acetate, and ethanol extracts and analyzed the antimicrobial, antioxidant and antiproliferative activities of the cortex, leaf, and fruit of the Rhamnus cathartica L. plant from Almaty, Kazakhstan. Material and Method: The GC-MS method was used to evaluate the chemicals of Rhamnus cathartica L. cortex, leaf, and fruit extracts. The microdilution technique was used to evaluate the MIC values of plant extracts against pathogenic microorganisms. DPPH, FRAP, and Total Flavonoid Content were evaluated for antioxidant activity. The antiproliferative effect of the extracts on MCF-7, A549, C6 and WI-38 cells was measured by XTT assay. Result and Discussion: According to GC-MS results, Trans-2-Decenal had the highest proportion at 2.88%. Eicosane is second at 1.71%. DPPH, FRAP and flavonoid values showed that the fruit ethyl acetate extract (F-EtAc) had the strongest antioxidant activity. F-EtAc extract had the strongest anticancer activity according to the selectivity index table (WI-38/MCF-7: 12.67, WI-38/C6: 31.38, WI-38/A549: 8.55). All plant extracts had high MIC values against Staphylococcus aureus based on reference source values. The MIC value of cortex and leaf in ethyl acetate was 0.078 mg/ml, cortex and leaf extracts had strong antimicrobial activity (0.312 mg/ml) for Bacillus cereus, while fruits had weakly effective MIC values (Significant=MIC <0.1 mg/ml). According to the results of this study, it can be said that Rhamnus cathartica L. plant extracts have an active content in terms of biological properties.
Keywords : Antimikrobiyal, antioksidan, antiproliferatif, Rhamnus cathartica

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