Electrochemical Comparison of the Interaction of 5-Nitrouracil with Single- or Double-Stranded DNA at mercury and glassy carbon electrodes

Authors : Mohamed Sayed IBRAHİM, Hossieny Sameh Mohamed IBRAHİM, Moustafa Mohamed KAMAL, Yassin Mohamed TEMERK

Pages : 1-8

Doi:10.5505/apjes.2014.46855

View : 12 | Download : 10

Publication Date : 2014-04-01

Article Type : Research Paper

Abstract :The interaction of the 5-Nitrouracil insert ignore into journalissuearticles values(5NU);, with ss-, and ds-DNA was investigated electrochemically in absence and presence of copper ions by using cyclic voltammetry insert ignore into journalissuearticles values(CV); and differential pulse stripping voltammetry insert ignore into journalissuearticles values(DPSV); at hanging mercury drop electrode insert ignore into journalissuearticles values(HMDE); and glassy carbon electrode insert ignore into journalissuearticles values(GCE); surfaces. It was found that, in absence of copper ions, the addition of ss- or ds-DNA to a buffered solution of 5NU results in a decrease on the 5NU redox peak current with a remarkable change in the peak potential insert ignore into journalissuearticles values(ca. 150 mV vs. Ag/AgCl); at both electrodes. This means that, an interaction of 5NU molecules with both ss- and ds-DNA was observed. The results also demonstrate that a distinguish between ss-, and ds-DNA can be achieved in presence of copper ion through their interaction with 5NU. The binding constants of 5NU with ds-DNA at HMDE and GCE were determined through the changes on the 5NU redox peak currents insert ignore into journalissuearticles values(at HMDE, 1.45x105 and at GCE, 2.65x105);. The calibration plot for the DNA determination was obtained through the corresponding decreases on the DPSV peak current of 5NU to different additions of DNA insert ignore into journalissuearticles values(ss- or ds-DNA); concentration levels at the optimum conditions.
Keywords : Voltammetry, 5 nitrouracil, interactions, DNA