Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Authors : Muhammet DOGAN

Pages : 4-8

Doi:10.30616/ajb.343304

View : 31 | Download : 9

Publication Date : 2017-10-25

Article Type : Research Paper

Abstract :Rotala rotundifolia insert ignore into journalissuearticles values(Buch-Ham. ex Roxb); Koehne, an aquatic plant belonging to the family Lythraceae, is used for the treatment of some diseases due to its medical and anti-microbial properties. This study presents multiple shoot regeneration from shoot tip and nodal explants of R. rotundifolia cultured on Murashige and Skoog insert ignore into journalissuearticles values(MS); nutrient medium containing 0.05-1.25 mg/L Kinetin insert ignore into journalissuearticles values(KIN); and 0.25 mg/L Gibberellic acid insert ignore into journalissuearticles values(GA 3 ); combinations for eight weeks. At the end of the second week, shoot formations began to be observed on the explants. High shoot regeneration frequencies were determined for both explants in the culture medium. The maximum number of shoots per explant was obtained from shoot tip insert ignore into journalissuearticles values(38.66); and nodal insert ignore into journalissuearticles values(30.77); explants cultured on MS medium containing 0.25 mg/L KIN + 0.25 mg/L GA 3 . Whereas the minimum number of shoots per explant was determined on MS medium containing 1.25 mg/L KIN + 0.25 mg/L GA 3 for both explant types. The highest shoot lengths for shoot tip insert ignore into journalissuearticles values(1.87 cm); and nodal insert ignore into journalissuearticles values(1.79 cm); explants were obtained on MS culture medium containing 0.75 mg/L KIN + 0.25 mg/L GA 3 and 0.50 mg/L KIN + 0.25 mg/L GA 3 , respectively. For in vitro rooting of the regenerated shoots, 2 cm long cut shoots were transferred to MS medium containing 0.25 mg/L indole-3-butyric acid insert ignore into journalissuearticles values(IBA);. The rooted shoots were then successfully acclimatized to external conditions in the aquarium environment. 
Keywords : In vitro, Kinetin, R rotundifolia, Shoot regeneration, Tissue culture

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