Effects of thymoquinone on valproic acid-induced oxidative stress in perinatal rat brain

Authors : Süeda TUNÇAK, Büşra ESMERCE, Birnur AYDİN, Bülent GÖREN

Pages : 76-81

Doi:10.30616/ajb.1254803

View : 53 | Download : 7

Publication Date : 2023-05-15

Article Type : Research Paper

Abstract :Thymoquinone insert ignore into journalissuearticles values(TQ);, bioactive molecule of black cumin, has antioxidant and neuroprotective effects. TQ’s hypoglecemic effect while applied prenatally is reported. This study is aimed to find the TQ dose with maximum antioxidant and minimum side effects in valproic acid insert ignore into journalissuearticles values(VPA); induced oxidative stress. Pregnant Wistar rats were injected i.p. with 400 mg/kg/ml of VPA on embryonic day 12.5 insert ignore into journalissuearticles values(E12.5);. Repeated dose groups were injected i.p. from E11.5- E14.5; RC- repeated control: did not receive TQ, R1: 0.5 mg/kg/ml of TQ, R2: 2 mg/kg/ml of TQ, R3: 4 mg/kg/ml of TQ, R4: 8 mg/kg/ml of TQ. Single dose groups were injected i.p. on E12.5; SC- single control: did not receive TQ, S1: 8 mg/kg/ml of TQ, S2: 15 mg/kg/ml of TQ. Pups were sacrificed on postnatal day 7. Glutathione insert ignore into journalissuearticles values(GSH);, malondialdehyde insert ignore into journalissuearticles values(MDA); and superoxide dismutase insert ignore into journalissuearticles values(SOD); levels were measured via ELISA method. Prenatal VPA exposure decreased GSH and SOD levels in RC and SC compared to naïve group. R3 group showed improved GSH and SOD levels compared to RC. No significant difference in MDA levels was found between groups. Antioxidant effects of TQ on VPA induced oxidative stress has been showed in R3 group. This dose can be used to investigate TQ’s effects on other parameters that are affected by prenatal VPA exposure.
Keywords : Oksidatif Stres, Timokinon, Valproik Asit, Perinatal Beyin, Sıçan

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