Genotoxic, Antigenotoxic and Antioxidant Properties of Methanol Extracts Obtained from Umbilicaria cylindirica and Umbilicaria nylanderiana

Authors : Derya EFE, Gökçe KARADAYI, Güleray AĞAR, Medine GÜLLÜCE, Ali ASLAN

Pages : 74-83

View : 14 | Download : 13

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :The use of lichens as food, medicine in dyes and perfumes and crude drugs is very common all over the world. It is so important to determine whether their use is safe or not and which doses should be used. In the present study, the genotoxic and antigenotoxic effects of methanol extracts of Umbilicaria cylindirica and Umbilicaria nylanderiana were investigated by using sister chromatid exchange in human lymphocytes against Aflatoxin B1 insert ignore into journalissuearticles values(AFB1); and the bacterial reverse mutation assay in Salmonella typhimurium TA1535 and TA1537 strains and in Escherichia coli WP2 uvrA strain against Sodium azide insert ignore into journalissuearticles values(NaN3);, 9-Aminoacridine insert ignore into journalissuearticles values(9-AA); and N-Methyl-N’-nitro-N-nitrosoguanidine insert ignore into journalissuearticles values(MNNG);, respectively. Furthermore, in order to evaluate antioxidant effect of the tested materials, the main antioxidant enzymes including superoxide dismutase insert ignore into journalissuearticles values(SOD);, glutathione insert ignore into journalissuearticles values(GSH); and glutathione peroxidase insert ignore into journalissuearticles values(GPX); and malondialdehyde insert ignore into journalissuearticles values(MDA); level were determined. At the end of the study, it was observed that Umbilicaria cylindirica and Umbilicaria nylanderiana exhibit no mutagenic activity, but significantly antimutagenic activity against all the different doses of mutagens and antioxidative activity against oxidative stress caused by AFB1.
Keywords : antimutagenicity, antioxidative, bacterial reverse mutation assay, SCE, Umbilicaria spp,