Spirulina (Arthrospira platensis) Extract Promotes Motility, Microscopic, and Antioxidative Parameters of Ram Semen during Refrigerated Storage

Authors : Serge KAMENİ LEUGOUÉ, Dongmo Nguedıa ARİUS BAULLAND, Tebug THOMAS TUMASANG, Bomba Tatsınkou FRANCİS DESİRE, Félix MEUTCHİEYE, Ngoula FERDİNAND

Pages : 481-490

Doi:10.47115/bsagriculture.1162523

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Publication Date : 2022-10-01

Article Type : Research Paper

Abstract :This study investigated the effect of spirulina ethanolic extract insert ignore into journalissuearticles values(SEE); on the quality of ram semen during low-temperature liquid storage and the relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk insert ignore into journalissuearticles values(TEY); enriched with 0 insert ignore into journalissuearticles values(control);, 20 insert ignore into journalissuearticles values(SEE20);, 40 insert ignore into journalissuearticles values(SEE40);, and 80 µg/mL insert ignore into journalissuearticles values(SEE80); of SEE to reach the concentration of 200×106 spz/mL, and stored at 4 °C for 72 h. Extended semen samples were assessed for total motility, progressive motility, sperm motion characteristics, viability, membrane integrity, and morphology at 6, 24, 48 and 72 h of storage. Moreover, malondialdehyde insert ignore into journalissuearticles values(MDA);, nitric oxide insert ignore into journalissuearticles values(NO);, superoxide dismutase, insert ignore into journalissuearticles values(SOD); and catalase insert ignore into journalissuearticles values(CAT); levels were measured at 72 h of storage. The enrichment of TEY with SEE at 40 and 80 µg/mL, improved sperm total motility at 48 and 72 h of storage insert ignore into journalissuearticles values(P<0.05);. Also, all SEE-treated samples evidenced higher progressive motility in comparison to the control at 48 and 72 h insert ignore into journalissuearticles values(P<0.05);. SEE80 group showed the highest percentages of viability insert ignore into journalissuearticles values(76.26±0.90%); and membrane integrity insert ignore into journalissuearticles values(58.19±1.50%);; whereas, SEE40 demonstrated the lowest percentage of morphological abnormality insert ignore into journalissuearticles values(18.14±1.01%); at 72 h of storage. SEE did not influence NO levels; however, at 40 µg/mL, it reduced MDA concentration and improved SOD and CAT activities insert ignore into journalissuearticles values(P < 0.05);. Total motility was positively correlated to progressive motility insert ignore into journalissuearticles values(r=0.69, P<0.01);, viability insert ignore into journalissuearticles values(r=0.91, P<0.01);, and membrane integrity insert ignore into journalissuearticles values(r=0.49, P<0.05);; while, morphological abnormality was negatively correlated to the other sperm parameters. Furthermore, MDA was negatively correlated to total motility insert ignore into journalissuearticles values(r=-0.91, P<0.01);, progressive motility insert ignore into journalissuearticles values(r=-0.70, P<0.01);, and viability insert ignore into journalissuearticles values(r=-0.91, P<0.01);, and positively correlated to morphological abnormality insert ignore into journalissuearticles values(r=0.70, P<0.01);. An entirely opposite figure was recorded for SOD. Overall, the results indicated that SEE, especially at 40 µg/mL, can protect ram semen against liquid storage-associated damages. Furthermore, positive and negative correlations exist between semen parameters.
Keywords : Liquid storage, Ram semen, Spirulina, Semen quality