In vitro Micropropagation of Maintainer White Head Cabbage Lines Using Cotyledon And Hypocotyl Explants

Authors : Senay MURAT DOĞRU, Ahmet BALKAYA, Ertan Sait KURTAR

Pages : 180-188

Doi:10.47115/bsagriculture.1088157

View : 21 | Download : 8

Publication Date : 2022-07-01

Article Type : Research Paper

Abstract :Brassica species are the most widely cultivated vegetable crops and improvement programs started in the last thirty years in Türkiye. Hybrid seed production is very difficult for Brassica vegetable species. Because the production of a new F1 hybrid cultivar needs a male sterile line insert ignore into journalissuearticles values(A);, maintainer line insert ignore into journalissuearticles values(B);, and also a male line insert ignore into journalissuearticles values(C);. Biotechnological methods provide an excellent opportunity for new F1 hybrid cultivar improvement via in vitro maintenance of the breeding lines. Thus, in vitro propagation possibilities of Matsunami F1 cultivar and 3 white head cabbage maintainer inbred lines were examined using hypocotyl and cotyledon explants. The most successful results were obtained from MS + 2 mg/l and 4 mg/l BAP combinations. Matsunami F1 was prominent in terms of proliferation rate. Root formation was found to be considerably high in all genotypes. The use of in vitro propagation techniques is expected to provide significant benefits in head cabbage breeding programs.
Keywords : Cabbage breeding, Explant, in vitro regeneration, Maintainer lines