Effect of Native Beauveria bassiana Vuillemin Isolates on Egg Hatching of Tetranychus urticae Koch (Acari: Tetranychidae)

Authors : Asiye UZUN YİĞİT

Pages : 16-20

Doi:10.47115/bsagriculture.1172258

View : 26 | Download : 10

Publication Date : 2023-01-01

Article Type : Research Paper

Abstract :In this study, the effect of culture filtrates prepared at different doses of native Beauveria bassiana isolates insert ignore into journalissuearticles values(BIM-001, BY2, and IGÇ); on egg hatching of Tetranychus urticae Koch was determined. The adult females of T. urticae were transferred to bean leaves insert ignore into journalissuearticles values(4 cm); prepared according to the leaf disc method, as 10 individuals. After 24 hours, 20 eggs/leaf disc were prepared in each petri dish. Pure culture filtrates insert ignore into journalissuearticles values(1X); and other diluted doses insert ignore into journalissuearticles values(5X, 10X); were applied to leaf discs containing eggs for 10 seconds by spraying method. Observations were started 24 hours after the application and continued until the 7th day. Experiments were carried out with 5 replications for each dose of entomopathogen fungus isolates. The egg hatching of T. urticae was 19% at the pure culture filtrate dose of B. bassiana BIM-001 isolate insert ignore into journalissuearticles values(1X); 7 days after the application, and it was different and significant than the other isolates insert ignore into journalissuearticles values(P < 0.05);. Egg hatching rates of T. urticae for BIM-001, BY2, and IGÇ isolates were determined between 19-38%, 32-48%, and 36-53%, respectively. These rates were found to be 31-38%, 43-48%, and 46-53% at 5X and 10X doses of BIM-001, BY2, and IGÇ isolates. There was no significant difference in egg hatching rates of pure culture filtrates of B. bassiana BY2 and IGÇ isolates insert ignore into journalissuearticles values(P˃0.05);.
Keywords : Culture filtrate, Entomopathogenic fungus, Two spotted spider mite